Isolation of chlamydia in irradiated and non-irradiated McCoy cells.

Growth of Chlamydia trachomatis in McCoy cells treated with cytochalasin B.

When the fungal metabolite cytochalasin B was added to McCoy cells, multinucleated giant cells developed. Monolayers of these cells proved as efficient as irradiated cells for the growth of three different serotypes of Chlamydia trachomatis and for the primary isolation of chlamydiae from clinical specimens obtained from patients attending a venereal disease clinic. Cytochalasin treatment of Mc...

Effect of high-speed centrifugation on the sensitivity of irradiated McCoy cell culture for the isolation of Chlamydia.

as well as Rickettsia prowazeki onto cell monolayers will increase the sensitivity of cell culture several fold. Using centrifugal force to facilitate the precipitation of infective particles onto irradiated McCoy cells, Gordon, Magruder, Quan, and Arm (1963) and Gordon, Dressler, and Quan (1967) developed a cell culture technique as an alternative to cultivation in the yolk sac of fertile eggs...

Isolation of Chlamydia spp. from Ewes and Does in Iran

Enzootic ovine abortion is caused by Chlamydia abortus and may result in abortion among small ruminants during the last 2-3 weeks of pregnancy. Enzootic abortion is diagnosed by isolation of the agent or detection of its nucleic acid in the products of abortion or vaginal excretions of freshly aborted females. Isolation of chlamydial agents in cell culture is the gold standard, so in the presen...

Isolation of TRIC agent (Chlamydia) in irradiated McCoy cell culture from endemic trachoma in field studies in Iran. Comparison with other laboratory tests for detection of Chlamydia.

Isolation of TRIC

The technique of cultivation ofTRIC agent, and other Chlamydia (Bedsonia) by centrifugation onto irradiated McCoy cells, developed by Gordon, Magruder, Quan, and Arm (i 963), Magruder, Gordon, Quan, and Dressler (i963), and Gordon, Dressler, and Quan (I967), has been used to recover laboratory-adapted isolates of TRIC agent from the infected eyes of simians (Gordon and others, I963) and of man ...